ProteaImmun provides activity analysis by zymography adapted for MMPs. Please contact our technical support for more information.

Zymography is a useful qualitative tool for detection and analysis of the level and type of the proteases expressed in different cell types/tissues at any given time and/or after different treatments. This technique, known mostly as gelatine/casein zymography, identifies enzymes activity in biological samples using sodium dodecyl sulfate (SDS)-polyacrylamide gels impregnated (copolymerized) with gelatine/casein [1]. To maintain enzymatic activity, the samples are electrophoresed under nonreducing conditions. After removal of the SDS from the gel by Triton X-100 and incubation in a calciumcontaining buffer, the partially renatured enzymes can degrade the copolymerized substrate, leaving a cleared zone that can be detected after staining of the gel. In the presence of SDS, the enzymes are denatured exposing their active site, which permits both the latent and active forms of MMPs to exhibit its activity after the partial renaturation [1].