FAQs - ADAMTS
Here you find frequently asked questions about ProteaImmun’s ADAMTS proteins and antibodies.
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Is there any description how to determine the inhibitory potential of chemicals on the activity of ADAMTS4 when using the kit?
We don’t have any description. But, you can follow the same procedure for standard curves of proteolytic reactions and for determination of inhibitory potential of chemicals on the activity of ADAMTS4. The difference is that you add the inhibitory chemicals to the test sample. A comparison of both reactions should appear in different activity values.
Is the activity different among aggrecanases?
Is known, also from our experience, that the activity of aggrecanases is very different (in terms of efficiency of aggrecan cleavage). For example, we know that ADAMTS1 is 3 - 10 times less active in cleaving aggrecan then ADAMTS4.
Can thrombospondin be cleaved by ADAMTS1?
First, be aware that our ADAMTS1 is a truncated form that comprises only the first thrombospondin domain. To demonstrate that truncated ADAMTS1 has lower activity for TSP1 then the full length, TSP1 was incubated with the full-length active ADAMTS1 (87 kDa) and with truncated active ADAMTS1 (65 kDa), which lacks part of the spacer region and the last two TSRs. The conclusion was that the truncated form of ADAMTS1 required a higher E:S (2:1) to achieve near complete processing of intact TSP1 within 2 h in comparison to 87 kDa ADAMTS1, which completely processed intact TSP1 at an E:S of 1:10 (ADAMTS1 mediates the release of antiangiogenic polypeptides from TSP1 and 2, EMBO J., 2006;25(22):5270-83).
Describe in more detail where are the truncations exactly located (amino acid location at the protein) for your truncated ADAMTS-4 and -5.
Our ADAMTS-5 protein is missing amino acids # 625 - 930, ADAMTS-4 consists of amino acids F213 - A579. The information is also given on the datasheets that were included in our web site.