FAQs - MMP-13 ELISA
Here you find frequently asked questions about ProteaImmun’s MMP13 assays.
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We need to know whether the act MMP-13 kit measures only active form of enzyme present in the body or it first converts latent form into active form and then measures overall MMP level (i.e. active and latent, which is activated before measuremen
The assay is specific for the activated form of collagenase 3. However, when present at equal concentrations, latent MMP 13 yields about 1/10 the OD-value of active MMP 13. As you already know, the activation of latent MMP13 is possible with APMA (p-amino-phenyl mercuric acetate) solution (and is followed by the incubation of the mixture for 90 minutes at 37 °C). Theoretically, one can make two different experiments: first as indicated in the kit manual to determine the active MMP13 and the second, after activation with APMA, to get the total (active + latent) MMP13 from serum.
Does your act MMP-13 kit contain Mercury and how much?
There is mercury in our MMP-13 standard. For the standard we activate pro-MMP13 with p-aminophenyl mercuric acetate (APMA). The final concentration is 0.3 µM after reconstitution of MMP-13 standard with 1 ml distilled water.
Is ProteaDetect® Human act MMP-13 (Collagenase 3) kit also appropriated to be used with rat MMP-13?
No it isn’t, MMP-13 ELISA has not been tested for animal species, so we don’t know if it will work with rat samples.
Has the ELISA for activated MMP-13 also been validated to be used with human synovial fluid specimens?
Yes. You can find further instructions in our ProteaDetect® Act-MMP13-Manual.
How can be determined the maximum amount of a pro-MMP-13 preparation that was tested?
When latent MMP 13 (Pro-MMP13) and active MMP13 are assayed in parallel with the ProteaDetect® Act MMP 13 kit, the proform must be present at about 10-fold higher concentrations to give the same OD-values as the active form. For example, when 0.2 nM active MMP13 give an OD-value of 0.4, with pro-MMP13 the same OD-value is reached only at a concentration of 2 nM.